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Working and learning notes on bioinformatics, genomics, sequencing, and computation.

Bhargava Reddy
Morampalli

Bioinformatics, from the wet lab to the command line.

Notes from the bench and the terminal: practical guides, working notes, and lessons from learning computation as a wet-lab scientist.

Currently Finishing and submitting my PhD thesis: identifying and comparing RNA modifications in natural isolates of Escherichia coli using Oxford Nanopore sequencing.

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LATEST

11 June 2026 23 min

Creating nf-core-style Nextflow modules
Bioinformatics Tools & Workflows
A practical guide to writing local Nextflow modules with modern nf-core-style metadata, topic-channel version outputs, stub tests, and nf-test snapshots.
1 June 2026 14 min

Checking bioinformatics files before you trust them
Bioinformatics Tools & Workflows
A reference for the command-line checks you run before trusting a file, launching a pipeline, or debugging a result.
3 April 2019 3 min

Python for Biological Data Analysis, Part 2: Working with Files
Python & Programming
How to read, write, and append biological sequence files in Python using safe file I/O patterns.
2 April 2019 3 min

Python for Biological Data Analysis, Part 1: Strings and Sequence Basics
Python & Programming
A practical introduction to Python string operations for biological sequence analysis.
4 March 2019 3 min

Extracting Mapped and Unmapped Reads from FASTQ
Bioinformatics Tools & Workflows
A practical command-line workflow to separate mapped and unmapped reads and recover them into FASTQ files.

Full archive →

Bioinformatics, from the wet lab to the command line.

Creating nf-core-style Nextflow modules

Checking bioinformatics files before you trust them

Python for Biological Data Analysis, Part 2: Working with Files

Python for Biological Data Analysis, Part 1: Strings and Sequence Basics

Extracting Mapped and Unmapped Reads from FASTQ