From Bench to Binary:
Bridging Molecular Biology and Bioinformatics
I am a scientist with over 10 years of research experience with a combination of both wet lab expertise and biological data analysis to solve complex biological problems.
Expertise Areas
Laboratory Skills
- Cell-based assay development
- Sequencing library preparation for nanopore sequencing - DNA, cDNA, direct RNA
- DNA/RNA isolation and QC
- Cloning and Site-directed mutagenesis
- PCR and qPCR (assay optimization)
- Western Blots and ELISA
- Immunofluorescence and Immunohistochemistry (IHC)
- Fluorescence microscopy and High content imaging (Perking Elmer)
Computational Skills
- NGS data analysis
- Nanopore/longread sequencing data analysis
- DNA sequencing data for genome assemblies
- direct RNA sequencing data for identifying modifications and transcriptomics analyses
- Illumina/short read sequencing data analysis
- RNA sequencing data analysis
- DNA sequencing for use in generating hybrid genome assemblies
- Nanopore/longread sequencing data analysis
- Python scripting
- data wrangling using pandas library
- data visualization using matplotlib, seaborn
- Jupyter notebooks for documentation and analysis
- scripts for automation of complex and routine tasks
- BASH scripting
- Nextflow for writing pipelines for reproducible analyses
- NGS data analysis